2019  (Bio-Techne corporation)

Journal: iScience

Article Title: Atmospheric particulate matter impairs pulmonary barriers by triggering FTH1-mediated ferroptosis

doi: 10.1016/j.isci.2026.115177

Figure Lengend Snippet: PM2.5 triggered ferroptosis and promoted inflammation in MH-S cells (A) Immunofluorescence analysis and quantification of CD68 expression (white arrows) in the lung tissues of mice subjected to various exposure patterns. Scale bars: 50 μm. Red arrows highlight the particle aggregates. (B) Confocal laser scanning microscopy images illustrating the phagocytic capacity of MH-S following PM2.5 exposure at various time intervals. Yellow arrowheads denote particle aggregates, and white arrowheads indicate filopodia protrusions. Scale bars: 50 μm. (C) The average number of pHrodo green Zymosan bioparticles ingested per cell. (D) The percentage of cells containing pHrodo green Zymosan bioparticles. (E–G) Volcano plots, KEGG enrichment, and GO analysis of DEGs in macrophages from PM2.5 exposure and Control groups in a dataset ( GSE294723 ). (H) Level of MDA in MH-S treated with PM2.5 (20 and 50 μg/cm 2 ) with or without the addition of Fer-1 (5 μM) for 6 h. (I) qPCR analysis of GPX4, SLC7A11, Nrf2, and SOD2. (J and K) IL-6 and TNF-α levels in MH-S detected by ELISA. (L–N) Immunofluorescence images and quantitative assessments of the phagocytic capacity of MH-S treated with PM2.5 (50 μg/cm 2 ) with or without Fer-1 (5 μM) for 6 h. Scale bars: 50 μm. Data are presented as mean ± SEM ( n = 4 independent experiments). ∗ p < 0.05 and ∗∗ p < 0.01 as determined by two-way ANOVA followed by Dunnett’s multiple comparison test.

Article Snippet: Mouse alveolar macrophage MH-S cells (ATCC, CRL-2019) were maintained in RPMI-1640 medium supplemented with 10% FBS (ExCell Bio, Suzhou, China).

Techniques: Immunofluorescence, Expressing, Confocal Laser Scanning Microscopy, Control, Enzyme-linked Immunosorbent Assay, Comparison

Journal: iScience

Article Title: Atmospheric particulate matter impairs pulmonary barriers by triggering FTH1-mediated ferroptosis

doi: 10.1016/j.isci.2026.115177

Figure Lengend Snippet: PM2.5 resulted in ferroptosis by upregulating FTH1 in BEAS-2B and MH-S (A) Hub DEGs were identified from the PPI network using Cytoscape, based on the GSE155616 dataset. (B and C) Western blot analysis of FTH1 in PM2.5-induced BEAS-2B and MH-S with or without Fer-1 addition. (C–E) Flow cytometry results of intracellular ROS of BEAS-2B and MH-S cells. (F and G) Western blot analysis and quantitative results of GPX4, SLC7A11, and HO-1 of BEAS-2B cells. (H and I) Western blot analysis and quantitative results of GPX4, SLC7A11, and HO-1 of MH-S cells. (J and K) Western blot analysis of ZO-1, occludin, and E-cadherin in BEAS-2B. Data are presented as mean ± SEM ( n = 3 independent experiments). ∗ p < 0.05 and ∗∗ p < 0.01 as determined by two-way ANOVA followed by Dunnett’s multiple comparison test.

Article Snippet: Mouse alveolar macrophage MH-S cells (ATCC, CRL-2019) were maintained in RPMI-1640 medium supplemented with 10% FBS (ExCell Bio, Suzhou, China).

Techniques: Western Blot, Flow Cytometry, Comparison